[P3-2-02] The analysis for the regulatory roles of Sake Lees on bone metabolisms
Keywords:骨粗鬆症、骨代謝、酒粕
In order to maintain healthy bones, it is important to balance the activity of osteoclasts and osteoblasts. In Japan, the number of osteoporosis patients is estimated to be about 10% of the total population. Although therapeutic agents have been applied to treat this disease, problems due to long-term administration have been pointed out. This study aims to explore and develop food ingredients that can prevent osteoporosis. We have investigated the effects of Sake Lees (SL) or Sake Lees Extract (SLE) on osteoblastic and osteoclastic differentiation and osteoporotic bone metabolism. Preosteoblastic cells (MC3T3-E1) were cultured in the presence or absence of SLE. Osteoblastic differentiation was assessed by alkaline phosphatase (ALP) staining, picrosirius red staining, hydroxyproline assay, alizarin red S staining, and Western blot analysis. SLE significantly increased ALP levels, collagen production, maturation, and mineralization in MC3T3-E1 cells. Also, the effect of SLE on osteoclastic differentiation was determined by using preosteoclastic cells (RAW264.7). Evaluation by TRAP staining revealed that SLE significantly inhibited osteoclastic differentiation. For the in vivo study, 12-week-old female C3H/HeJ mice were used. They were ovariectomized (OVX) or sham-operated and fed normal food or food containing 20% SL, 40% SL, or isoflavones, as a positive control, for 4 weeks. Micro-CT analysis was performed to evaluate the bone microstructure of the distal femur. Compared to OVX mice fed the normal diet, mice fed SL (20% and 40%) showed a slight increase in trabecular number and statistically significant increases in trabecular bone volume and trabecular bone thickness. These results suggest that the intake of SL maintains bone mass in osteoporotic trabecular bone. Hence, this study revealed that sake lees have a beneficial effect on the maintenance of trabecular bone in osteoporosis through activating osteoblasts and inhibiting osteoclasts.