[P3-3-06] Effect of allopurinol, a xanthine oxidase inhibitor, on cardiac dysfunction caused by occlusal disharmony
Keywords:咬合不調和、酸化ストレス、心疾患
Objective: Chronic stress due to occlusal disharmony has been suggested as a possible trigger for cardiac disease, but the details of the molecular mechanism are unknown. We have previously established a bite-opening (BO) mice, in which a 0.7 mm space was introduced by cementing a suitable appliance onto the mandibular incisor under anesthesia. In this study, we tested whether xanthine oxidase inhibitor (allopurinol; ALLO) could attenuate cardiac dysfunction in mice treated with BO for 2 weeks. Methods: C57BL/6J mice (male, 16 weeks old) were divided into four groups: a normal control group (Control), a BO-only treatment group (BO), a allopurinol -only treatment group (ALLO), and a BO plus allopurinol treatment group (BO + ALLO) (Control group: n = 6, BO group: n = 8, ALLO group: n = 6, BO+ALLO group: n = 8). Two weeks after the start of BO treatment, cardiac function (left ventricular ejection fraction and fractional shortening) was measured by echocardiography. After cardiac function measurements, each organ (heart, skeletal muscle, liver, and lungs) was removed and weighed. Results: Compared to the control group, the BO group showed lower cardiac function, and the ALLO group showed a tendency to suppress the decline in cardiac function caused by BO treatment. We examined cardiac function by echocardiography and found that cardiac function was significantly decreased in PG-LPS-treated group. Conclusion: Allopurinol was suggested to have a preventive effect against cardiac disease caused by occlusal disharmony.