[HIRANUMA-5] Biological evaluation and drug releasing aspect of graphene oxide coated zirconia
[Abstract]
Objective:
To determine the effects of plasma-based graphene oxide on bacterial adhesion and osteoblast activation. and the role as a drug carrier.
Material and methods:
An atmospheric pressure plasma generator was used to coat graphene oxide onto zirconia specimens. The groups were divided into uncoated (control; Zr) group and GO-coated (Zr-GO) group. The surface characteristics(FE-SEM, Raman spectroscopy) and biological aspects(crystal violet assay, WST-8, ALP assay) were evaluated. Drug release evaluation was performed using ultraviolet visible light spectroscopy. For statistical analyses, the Mann-Whitney U test was performed.
Result:
FE-SEM and Raman spectroscopy demonstrated effective Graphene oxide deposition. S. mutans was significantly reduced in Zr-GO group (P < 0.05). While no significant differences in cell attachment was observed, both proliferation and differentiation were increased in Zr-GO group (P < 0.05). In Drug releasing aspect, first 72 hours, Zr-GO group showed sustained drug release compared to the Zr group. However after 7 days, the total released amount of drug showed almost the same.
Conclusion:
Zirconia coated with graphene oxide inhibited the adhesion of S. mutans and increased the proliferation and differentiation of MC3T3 – E1 cell. The graphene oxide coated zirconia has shown the sustained release of dexamethasone compared to the zirconia with no treatment, .
トピックス
●Graphene oxide
●Non thermal atmospheric pressure plasma
●zirconia implant
Objective:
To determine the effects of plasma-based graphene oxide on bacterial adhesion and osteoblast activation. and the role as a drug carrier.
Material and methods:
An atmospheric pressure plasma generator was used to coat graphene oxide onto zirconia specimens. The groups were divided into uncoated (control; Zr) group and GO-coated (Zr-GO) group. The surface characteristics(FE-SEM, Raman spectroscopy) and biological aspects(crystal violet assay, WST-8, ALP assay) were evaluated. Drug release evaluation was performed using ultraviolet visible light spectroscopy. For statistical analyses, the Mann-Whitney U test was performed.
Result:
FE-SEM and Raman spectroscopy demonstrated effective Graphene oxide deposition. S. mutans was significantly reduced in Zr-GO group (P < 0.05). While no significant differences in cell attachment was observed, both proliferation and differentiation were increased in Zr-GO group (P < 0.05). In Drug releasing aspect, first 72 hours, Zr-GO group showed sustained drug release compared to the Zr group. However after 7 days, the total released amount of drug showed almost the same.
Conclusion:
Zirconia coated with graphene oxide inhibited the adhesion of S. mutans and increased the proliferation and differentiation of MC3T3 – E1 cell. The graphene oxide coated zirconia has shown the sustained release of dexamethasone compared to the zirconia with no treatment, .
トピックス
●Graphene oxide
●Non thermal atmospheric pressure plasma
●zirconia implant