2015年 第76回応用物理学会秋季学術講演会

講演情報

シンポジウム(口頭講演)

シンポジウム » English session: Asian Joint Symposium on Nanobiotechnology

[14a-3A-1~8] English session: Asian Joint Symposium on Nanobiotechnology

2015年9月14日(月) 09:00 〜 11:45 3A (国際会議室)

Chair:Eiichi Tamiya(Osaka Univ.),Atsushi Miura(Hokkaido Univ.)

10:00 〜 10:15

[14a-3A-4] Neuronal selective growth on nanopillars using supported lipid bilayer

〇Nahoko Kasai1, Akie Watanabe1, Roxana Filip1, Toichiro Goto1, Yoshiaki Kashimura1, Aya Tanaka1, Shingo Tsukada1, Koji Sumitomo1, John F. Ryan2, Hiroshi Nakashima1 (1.NTT BRL, 2.Univ. Oxford)

キーワード:nanopillar,neuron,supported lipid bilayer

Neuronal patterning is of interest for understanding signal propagation between neurons and neuron-matrix interactions and thus elucidating neuronal functions. It is also useful for cell-based assays. The patterning of living cells in vitro has been made possible thanks to surface topographic features and/or physicochemical properties. In this study, the neurons were successfully patterned on nanometer-scale pillars used as scaffolds using a supported lipid bilayer (SLB) covering a base substrate to avoid cellular attachment.
500nm-diameter nanopillars of amorphous silicon (a-Si) were fabricated on a quartz substrate using electron beam lithography. For neuronal growth selectivity towards the pillar, an SLB composed of 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) was formed using lipid self-spreading to avoid cell attachment. Neurons were prepared from rat cortex, and cultivated on the above nanopillar substrate with the SLB for 7-9 days. Neuronal growth was examined by fluorescent immunostaining using confocal microscopy and scanning electron microscopy (SEM).
We confirmed using a fluorescent microscope that the SLB was formed by self-spreading. Then SEM observation revealed that the neurons of 7DIV grew selectively on the nanopillars. However, the neurons grew randomly where no SLB was formed.
These results show that the non-fouling characteristic of an SLB is useful for patterning neurons on nanopillars used as scaffolds and for providing an in vitro biological environment in which to examine neuronal functions.