2016年 第77回応用物理学会秋季学術講演会

講演情報

一般セッション(口頭講演)

4 JSAP-OSA Joint Symposia 2016 » 4.2 Bio-and Medical Photonics

[15a-C31-1~9] 4.2 Bio-and Medical Photonics

2016年9月15日(木) 09:15 〜 12:15 C31 (日航3階孔雀AB)

西舘 泉(農工大)、藤田 克昌(阪大)

09:45 〜 10:15

[15a-C31-3] [JSAP-OSA Joint Symposia 2016 Invited Talk] Hybrid light microscopy methods for multi-dimensional imaging of small animal models

Nanguang Chen1、Yubo Duan1、Shilpa Pant1 (1.NUS)

キーワード:Light microscopy, Imaging speed, Optical sectioning

Light microscopy methods have been widely used in biomedical research. Different microscopy techniques have different advantages and disadvantages. For example, wide-field optical microscopy is known for its convenience and high imaging speed. However, it has no depth sectioning capability due to the missing axial high-frequency components in its 3D optical transfer function. It is therefore impossible to obtain 3D structures of specimen based on wide-field images stacks. Laser scanning microscopes (e.g., confocal microscopes), on the other hand, can provide the so-called optical sectioning capability and high axial resolution. While conventional confocal microscopy is a standard tool for 3D visualization of fixed samples, their slow scanning speed and excessive photobleaching make it less suitable for real-time in vivo imaging of live samples. We have developed a hybrid strategy to combine information information from both scanning microscopy and wide-field microscopy imaging modes. A prototype hybrid microscope has been designed and implemented. We have verified its imaging performances, such as reduced photobleaching, improved imaging speed, and improved optical sectioning, with imaging results acquired from a wide range of live samples.