09:00 〜 09:15
▲ [8a-A409-1] Measuring elasticity of HeLa cells by laser trapping
キーワード：laser trapping, HeLa cell, biological cell elasticity
The objective of our research is to develop the non-invasive method for measuring the elasticity of the biological cells. We developed the experimental setup which consists of optical microscope and laser trapping system. In order to avoid cell damage the NIR laser was used as trapping and detection laser.
The fluorescent 500 nm polystyrene spheres were immersed in the medium with HeLa cells. A single polystyrene sphere was trapped and moved to the membrane of the cell, after the cell was indent, the polystyrene sphere was moved away from the cell and this process was repeated.
For determining the elasticity of the cell, we need to find the value of the cell indentation. From the comparison of the displacements of the polystyrene sphere while indenting and not indenting the cell. When the polystyrene bead touched the cell, the time delay occurs, from which the indentation value is obtained.
To determine cell elasticity, the variation on the Hertz model was used. This model describes a deformation of a large elastic body with a disc shaped contact area.The cell elasticity for the HeLa cells was obtained. Ability to investigate the mechanical properties may lead to understanding cell functions and diseases, what opens new possibilities for novel treatments of biological cells.
The fluorescent 500 nm polystyrene spheres were immersed in the medium with HeLa cells. A single polystyrene sphere was trapped and moved to the membrane of the cell, after the cell was indent, the polystyrene sphere was moved away from the cell and this process was repeated.
For determining the elasticity of the cell, we need to find the value of the cell indentation. From the comparison of the displacements of the polystyrene sphere while indenting and not indenting the cell. When the polystyrene bead touched the cell, the time delay occurs, from which the indentation value is obtained.
To determine cell elasticity, the variation on the Hertz model was used. This model describes a deformation of a large elastic body with a disc shaped contact area.The cell elasticity for the HeLa cells was obtained. Ability to investigate the mechanical properties may lead to understanding cell functions and diseases, what opens new possibilities for novel treatments of biological cells.