[19a-F306-1~12] 12.7 Biomedical Engineering and Biochips

[19a-F306-12] Fluorescence detection of biomarkers using the pipette tip-type automatic immunosensing system

〇Eri Takano¹, Nobuaki Shimura², Toshifumi Takeuchi¹ (1.Kobe Univ., 2.System Instruments Co., Ltd.)

Keywords:Automated biosensing system, Fluoroimmunoassay, Biomarkers

A pipette tip-type automatic biosensing system was developed for the detection of biomarker proteins. The pipette tip was designed to be able to incorporate a micro-chip immobilized with molecular recognition elements. First, a gold thin layer was formed on the micro-chip by sputtering. Antibodies were then immobilized on the gold sputtered micro-chip via protein A. A micro-chip was inserted into the inside of the designed pipette tip and fluorescent sandwich assay for the target biomarker protein was carried out. All of the procedures including all the liquid handling and the detection of analytes were automatically performed with a single pipette tip. The quantitativeness of the target biomarker proteins was evaluated from the relationship between the fluorescence intensity and the analyte concentration. The fluorescence intensity increased with the concentration of the analyte biomarker protein, and approximately 1 ng ml^-1 of the analyte biomarker protein in the human serum was detected.