[ODP-107] Whole genome analysis of Staphylococcus aureus ST9 strains isolated in Myanmar
Background: Isolates belonging to sequence type 9 (ST9) are the most widespread livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in Asia.
Objective: To elucidate the genetic feature of S. aureus ST9 firstly isolated in Myanmar.
Methods: The whole genome sequence of ST9-MRSA (strain S286) was determined by the next generation sequencing, and analyzed for SNPs, mobile genetic elements, and resistance genes.
Results: S286 strain harbored 2.8 Mb of chromosomal DNA, and two plasmids. Phylogenetic analysis revealed that S286 clustered with S. aureus ST9 isolates from African, American, and European countries. The Staphylococcal cassette chromosome mec (SCCmec) unit in S286 comprised 2 sets of recombinase gene complex 1 (ccrAB1) and mec complex C2 (SCCmec type IX: 1C2&1). The region encompassing from the first ccrAB1 to the mec complex showed similarity to the identical region of ST9-MRSA-IX strain JCSC6690 from Thailand. plasmid-1 (6.5 kb) carried lincosamide resistance gene, vga (A), plasmid-2 (5.2 kb) possessed aminoglycoside resistance gene (aadE) and chloramphenicol resistance gene (cat). Both of the plasmids exhibited high homology to those found in LA-MRSA clonal complex (CC) 398, which is the most frequently detected LA-MRSA in Europe and North America. Full genome analysis of a methicillin-sensitive S. aureus ST9 strain from Myanmar is in progress.
Objective: To elucidate the genetic feature of S. aureus ST9 firstly isolated in Myanmar.
Methods: The whole genome sequence of ST9-MRSA (strain S286) was determined by the next generation sequencing, and analyzed for SNPs, mobile genetic elements, and resistance genes.
Results: S286 strain harbored 2.8 Mb of chromosomal DNA, and two plasmids. Phylogenetic analysis revealed that S286 clustered with S. aureus ST9 isolates from African, American, and European countries. The Staphylococcal cassette chromosome mec (SCCmec) unit in S286 comprised 2 sets of recombinase gene complex 1 (ccrAB1) and mec complex C2 (SCCmec type IX: 1C2&1). The region encompassing from the first ccrAB1 to the mec complex showed similarity to the identical region of ST9-MRSA-IX strain JCSC6690 from Thailand. plasmid-1 (6.5 kb) carried lincosamide resistance gene, vga (A), plasmid-2 (5.2 kb) possessed aminoglycoside resistance gene (aadE) and chloramphenicol resistance gene (cat). Both of the plasmids exhibited high homology to those found in LA-MRSA clonal complex (CC) 398, which is the most frequently detected LA-MRSA in Europe and North America. Full genome analysis of a methicillin-sensitive S. aureus ST9 strain from Myanmar is in progress.