The 95th Annual Meeting of Japanese Society for Bacteriology

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Wakate Colosseum for Bacteriology

[WCB] Joint Symposium: Wakate Colosseum for Bacteriology —Young bacteriological research for the future—

Tue. Mar 29, 2022 2:30 PM - 7:30 PM Channel 2

Conveners: Masatoshi Miyakoshi(University of Tsukuba), Rino Isshiki(Waseda University), Satoshi Shibata(Tottori University), Toyotaka Sato(Hokkaido University), Akira Fukuda(Rakuno Gakuen University)

[WCB-10] The assembly mechanism and structures of Type V pili

Satoshi Shibata1, Mikio Shoji3, Matthias Wolf2, Jyun Fujii1 (1Div. Bacteriol, Dept. Microbiol. Immunol., Fac. Med., Tottori Univ., 2Molecular Cryo-Electron Microscopy Unit, OIST, 3Dept. Microbiol. Oral Infec., Grad Sch Biomed Science, Nagasaki Univ.)

Adhesive pili are important for bacterial colonization, biofilm formation, and virulence. The class Bacteroidia bacteria including a periodontal pathogen Porphyromonas gingivalis, have unique pili that are categorized as the type V pilus. Recent our X-ray crystallography of monomer pilins of Bacteroidia bacteria and cryo-electron microscopy analysis of polymerized pilins of Fim pilus of P. gingivalis revealed that that the protease-mediated strand exchange of pilins is involved in the type V pilus polymerization at the cell surface. We present cryo-EM structures of other type V pili besides Fim pilus that are currently being analyzed and discuss the similarities and differences in those structures and polymerization mechanisms.