[WCB] 【共催】細菌学若手コロッセウム―未来を拓く若手細菌学研究―

[WCB-10] The assembly mechanism and structures of Type V pili

柴田 敏史¹，庄子 幹郎³，Matthias Wolf²，藤井 潤¹ (¹鳥取大・医・感染制御・細菌学，²生体分子電子顕微鏡解析ユニット・沖縄科学技術大学院大学，³長崎大・医歯薬学総合・口腔病原微生物)

Adhesive pili are important for bacterial colonization, biofilm formation, and virulence. The class Bacteroidia bacteria including a periodontal pathogen Porphyromonas gingivalis, have unique pili that are categorized as the type V pilus. Recent our X-ray crystallography of monomer pilins of Bacteroidia bacteria and cryo-electron microscopy analysis of polymerized pilins of Fim pilus of P. gingivalis revealed that that the protease-mediated strand exchange of pilins is involved in the type V pilus polymerization at the cell surface. We present cryo-EM structures of other type V pili besides Fim pilus that are currently being analyzed and discuss the similarities and differences in those structures and polymerization mechanisms.