[3Ea] 血糖調節、認知機能

[3Ea-08] Ergothioneine stimulates Ca²⁺ signaling-mediated brain-derived neurotrophic factor expression in NE-4C cell

*史 彩月¹、松井 利郎^1,2 (1. 九大・院・生資環、2. 九大院・農)

キーワード：エルゴチオネイン、脳由来神経栄養因子、アルツハイマー病、NE-4C神経細胞

【Objective】Ergothioneine (EGT) has been demonstrated to be protective of cognition in animal models and humans. The underlying mechanism, however, is still a mystery to be unveiled. Therefore, in current study, EGT-induced Ca²⁺ signaling-mediated BDNF expression was explored.【Method】In cell culture, the mouse NE-4C stem cell was firstly differentiated into progenitor cell and then treated with sample for 48 h. Finally, the CREB phosphorylation and BDNF expression were detected. Besides, the Ca²⁺ real-time change was monitored by multi-mode microplate reader.
【Result】EGT significantly increased CREB phosphorylation and BDNF expression in NE-4C nerve cells. The elevated intracellular Ca²⁺ was inhibited by 2-APB (an IP₃R inhibitor). The analogue hercynine also enhanced intracellular Ca²⁺, yet histidine has no such effect. This suggested the trimethylammonium group may exert crucial role in Ca²⁺ signaling.