11:15 AM - 11:30 AM
[3Ga-10] Effect of alcohol-based sanitizers on transdermal sensitization to egg white allergen (OVA)
Keywords:allergy, transdermal sensitization, egg white allergen (OVA), alcohol-based sanitizers
【Purpose】
Recently, transdermal sensitization (TS) has gained attention as the primary sensitization pathway for food allergy. This skin-mediated allergen invasion and sensitization involves disruption of the skin barrier and inflammation, and cytokines involved in inflammation and migration, such as TSLP and TARC, are also involved.Since the COVID-19 pandemic, the use of alcohol-based sanitizers (ABS) has increased in frequency. As a result, there is concern about an increase in health hazards such as skin irritation due to excessive degreasing and drying of the skin. Such ABS may have an adverse effect on skin barrier and are assumed to have an impact on antibody production and food allergy development through TS. In this study, we examined the effect of ABS on TS using ovolbumin (OVA), egg white allergen, as an indicator of the production of Th2-type antibodies (IgG1, IgE) to OVA, and examined the mechanism of this effect.
【Methods】
In this experiment, 70% isopropanol was used as ABS and was tested on BALB/c mice. Once a week, after shaving the back of the mice, tape stripping and application of 5% SDS were performed. Four times a week, water or alcohol was applied to the shaved area, and after drying, the area was exposed to OVA solution. Serum and skin samples of the applied area were collected for analysis. Analysis items included detection of OVA-specific IgG1 and IgE antibodies in serum by ELISA and Western blotting, and quantification of TSLP and TARC in skin extracts by ELISA.
【Results】
ELISA using serum at 3 weeks of treatment showed that IgG1/IgE levels were significantly lower in the alcohol group than in the water group. Therefore, it is suggested that ABS may rather inhibit TS of allergens. We plan to investigate the mechanism of inhibition.
Recently, transdermal sensitization (TS) has gained attention as the primary sensitization pathway for food allergy. This skin-mediated allergen invasion and sensitization involves disruption of the skin barrier and inflammation, and cytokines involved in inflammation and migration, such as TSLP and TARC, are also involved.Since the COVID-19 pandemic, the use of alcohol-based sanitizers (ABS) has increased in frequency. As a result, there is concern about an increase in health hazards such as skin irritation due to excessive degreasing and drying of the skin. Such ABS may have an adverse effect on skin barrier and are assumed to have an impact on antibody production and food allergy development through TS. In this study, we examined the effect of ABS on TS using ovolbumin (OVA), egg white allergen, as an indicator of the production of Th2-type antibodies (IgG1, IgE) to OVA, and examined the mechanism of this effect.
【Methods】
In this experiment, 70% isopropanol was used as ABS and was tested on BALB/c mice. Once a week, after shaving the back of the mice, tape stripping and application of 5% SDS were performed. Four times a week, water or alcohol was applied to the shaved area, and after drying, the area was exposed to OVA solution. Serum and skin samples of the applied area were collected for analysis. Analysis items included detection of OVA-specific IgG1 and IgE antibodies in serum by ELISA and Western blotting, and quantification of TSLP and TARC in skin extracts by ELISA.
【Results】
ELISA using serum at 3 weeks of treatment showed that IgG1/IgE levels were significantly lower in the alcohol group than in the water group. Therefore, it is suggested that ABS may rather inhibit TS of allergens. We plan to investigate the mechanism of inhibition.