Session 4

[BISC4-01] Optimization of All-Optical Physiology by Two-Photon Holographic Microscope for Avoiding Crosstalk Problem

*Priyanka Gore¹, Manoj Kumar², Naru Yoneda², Osamu Matoba², Mitsuhiro Morita^1,2 (1. Department of Biology, Kobe University Graduate School of Science, 2. Center of Optical Scattering imaging Science, Kobe University)

All-optical physiology to study neuronal network in the brain through Ca²⁺ imaging and optogenetic stimulation using two-photon holographic microscope was optimized for avoiding crosstalk, which is the artificial activation of opsins by imaging laser.