Session 7

[BISC7-06] Time-lapse imaging of tumor spheroid drug response by dynamic optical coherence tomography integrated with cell cultivation chamber

*Ibrahim Abd El-Sadek^1,3, Rion Morishita¹, Atsuko Furukawa², Masato Iwatsuki², Shuichi Makita¹, Pradipta Mukherjee¹, Satoshi Matsusaka², Yoshiaki Yasuno¹ (1. Computational Optics Group, University of Tsukuba, Japan, 2. Clinical Research and Regional Innovation, Faculty of Medicine, University of Tsukuba, Japan, 3. Department of Physics, Faculty of Science, Damietta University, Egypt)

We demonstrate time-lapse drug response imaging of tumor spheroids by integrating a spheroid cultivation chamber and dynamic optical coherence tomography (DOCT) microscope. This newly integrated system enables high-time-resolution imaging of a living sample. It successfully revealed the fine temporal and spatial responses of human breast cancer (MCF-7) spheroids to paclitaxel and doxorubicin.