BISC Poster Session

[BISCp-14] Phase measurements for plant cells using transport-of-intensity phase imaging under commercially available confocal microscopy

*Naru Yoneda^1,2, Takumi Tomoi^3,4, Joe Sakamoto^5,6, Yosuke Tamada^3,7,8, Osamu Matoba^1,2 (1. Graduate School of System Informatics, Department of System Science, Kobe University, 2. Center of Optical Scattering Image Science, Kobe University, 3. Faculty of Engineering, Utsunomiya University, 4. Center for Innovation Support, Institute for Social Innovation and Cooperation, Utsunomiya University, 5. Biophotonics Research Group, Exploratory Research Center on Life and Living Systems, 6. Division of Biophotonics, National Institute for Physiological Science, 7. Center for Optical Research and Education (CORE), Utsunomiya University, 8. Robotics, Engineering and Agriculture-Technology Laboratory (REAL), Utsunomiya University)

Quantitative phase imaging (QPI) based on transport of intensity equation (TIE) under commercially available confocal microscopy have been proposed. The proposed method uses bright field module attached in usual confocal microscopy. In this paper phase distributions of plant cells are measured by using TIE-based confocal microscopy. The experimental results indicate the proposed method can measure phase distributions of the moss Physcomitrium patens.