BISC Poster Session

[BISCp-23] Enhancing the Variation of Fluorescence Signal of qPCR System Through Two-Dimensional Visualization Image and Intelligent Image Analysis

Cheng-Ru Li¹, Liang-Chieh Chao¹, *Hsin-Yi Tsai¹, Yu-Hsuan Lin¹, Kuo-Cheng Huang¹, Dar-Bin Shieh² (1. Taiwan Instrument Research Institute, National Applied Research Laboratories, 2. Institute of Oral Medicine, School of Dentistry, National Cheng Kung University)

This study proposed the two-dimensional (2D) visualization image and intelligent image analysis method to determine the tiny fluorescence variation. The results showed that the sum of intensity above threshold had better resolution than average intensity.