09:50 〜 10:04
[WS4-01] Proteogenomic landscape of extracellular vesicles (EVs): functional insights and diagnostic potential
Secretion and exchange of extracellular vesicles (EVs) by most cell types is emerging as a central paradigm for intercellular communication. Although much is known about EVs there is still lack of definition as to how many naturally-occurring EV classes/subtypes exist and how their biochemical and functional properties differ; critical information if EVs are to be fully harnessed for therapeutic applications including precision medicine.
Two major, molecularly-distinct EV classes are well described: 30-1000 nm diameter shed microvesicles (sMVs, also known as microparticles, or ectosomes) and exosomes (30-150 nm diameter). Accruing evidence reveals subtypes within each EV class exist and are molecularly distinct. More recently, we discovered a third major class of EV - shed midbody remnants (sMB-Rs) - which is biochemically distinct from exosomes and sMVs.
Here, I will present a comparative proteomic and transcriptomic profiling analysis of three EV classes ( exosomes, sMVs and sMB-Rs) and discuss the diagnostic potential their cargo (protein/ RNA species (mRNA, pseudogene transcripts, miRNA, and lncRNA)/ fusion genes) in the context of disease biomarkers and precision medicine. Additionally, I will discuss our recent findings concerning the role of EVs in the tumour microenvironment (especially, exosome-dependent tumour-stromal communication), and H-RAS-induced epithelial-mesenchymal transition (EMT).
Two major, molecularly-distinct EV classes are well described: 30-1000 nm diameter shed microvesicles (sMVs, also known as microparticles, or ectosomes) and exosomes (30-150 nm diameter). Accruing evidence reveals subtypes within each EV class exist and are molecularly distinct. More recently, we discovered a third major class of EV - shed midbody remnants (sMB-Rs) - which is biochemically distinct from exosomes and sMVs.
Here, I will present a comparative proteomic and transcriptomic profiling analysis of three EV classes ( exosomes, sMVs and sMB-Rs) and discuss the diagnostic potential their cargo (protein/ RNA species (mRNA, pseudogene transcripts, miRNA, and lncRNA)/ fusion genes) in the context of disease biomarkers and precision medicine. Additionally, I will discuss our recent findings concerning the role of EVs in the tumour microenvironment (especially, exosome-dependent tumour-stromal communication), and H-RAS-induced epithelial-mesenchymal transition (EMT).