[3P-352_3SP-04-15] Application of chromosome engineering technology (2): I Improving the efficiency of microcell mediated chromosome transfer using small molecular compounds

^○Koya Tajima¹, Narumi Uno¹, Yasuhiro Kazuki^2,3,4, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.CERC, Tottori Univ., 3.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 4.Chr. Eng. Group, ExCELLS)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[3P-353_1SP-05-05] Application of chromosome engineering technology (3): Direct chromosome transfer method from human iPS cells to mouse ES cells for production of trans chromosomic mice containing a human chromosome.

^○Koki Kuroiwa¹, Hitomaru Miyamoto¹, Kyotaro Yamazaki², Chihiro Yoshimatsu¹, Narumi Uno³, Teruhiko Suzuki⁴, Kanako Kazuki⁵, Kazuma Tomizuka³, Yasuhiro Kazuki^1,2,5,6 (1.Div. of Chromosome Biomedical Engineering., Grad. Sch. of Med. Sci., Tottori Univ., 2.Chr. Eng. Group, ExCELLS., 3.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 4.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 5.CERC, Tottori Univ, 6.Sch. of Life Sci., Facul. of Med., Tottori Univ.)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[3P-354] Application of chromosome engineering technology (4):　Generation of trans-chromosomic mice by direct injection of microcells with mouse artificial chromosome

^○Eiji Mizutani¹, Hiroyuki Satofuka³, Kyotaro Yamazaki⁴, Takeshi Endo⁵, Mirei Matsuoka², Akane Okada³, Yasuhiro Kazuki^2,3,4 (1.Inst. of Med., Univ. of Tsukuba, 2.Sch. of Life Sci., Facul. of Med., Tottori Univ., 3.CERC, Tottori Univ., 4.Chr. Eng. Group, ExCELLS：ExCELLS, 5.Trans Chromosomics, Inc.)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[3P-355_2SP-04-03] Application of chromosome engineering technology (5):Development of a method for chromosome cloning from human iPS cells by genome editing

^○Hiroaki Kobayashi¹, Hitomaru Miyamoto², Yosuke Hiramuki³, Teruhiko Suzuki⁴, Yasuhiro Kazuki^3,5,1,2 (1.Sch. of Life Sci., Facul. of Med., Tottori Univ., 2.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 3.CERC, Tottori Univ., 4.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 5.Chr. Eng. Group, ExCELLS)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[3P-356] Application of chromosome engineering technology (9): Development of a new gene-loading system for artificial sequence assembly on HAC/MAC

^○Teruhiko Suzuki¹, Mana Yamakawa¹, Akane Okada², Kanako Kazuki², Satoshi Abe², Takahiko Hara^1,3,4, Yasuhiro Kazuki^2,5,6,7 (1.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 2.CERC, Tottori Univ., 3.Grad. Sch., Tokyo Med. Dent. Univ., 4.Grad. Sch., Tokyo Metropol. Univ., 5.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 6.Chr. Eng. Group, ExCELLS., 7.Sch. of Life Sci., Facul. of Med., Tottori Univ.)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[3P-357] Application of chromosome engineering technology (7): Decoding the centromere chromatin using human artificial chromosome

^○Junichiro Ohzeki¹, Kyotaro Yamazaki¹, Kazuto Kugou², Koei Okazaki², Megumi Nakano², Koichiro Otake², Kenta Shirasawa², Sachiko Isobe², Yusuke Endo², Hiroshi Masumoto², Yasuhiro Kazuki^1,3,4,5 (1.Chr. Eng. Group, ExCELLS, 2.Kazusa DNA Res. Inst., 3.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 4.CERC, Tottori Univ., 5.Sch. of Life Sci., Facul. of Med., Tottori Univ.)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[3P-358_3SP-05-01] Application of chromosome engineering technology (8):Identification of essential genomic regions using human iPS cells with partial monosomy of chromosome 21 generated by megabase chromosome deletions

^○Yusaku Okano², Rina Komazaki², Yuki Ishizu², Masaya Egawa², Yusuke Ohkame³, Chihiro Yoshimatsu¹, Kyotaro Yamazaki⁶, Teruhiko Suzuki⁴, Yasunori Aizawa⁵, Narumi Uno², Yasuhiro Kazuki^3,1,6, Kazuma Tomizuka² (1.Div. of Chromo. Biomed Eng., Grad. Sch. of Med. Sci., Tottori Univ., 2.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci.,, 3.CERC, Tottori Univ.,, 4.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci.,, 5.Sch. Life Sci. Tech., Tokyo Tech.,, 6.Chr. Eng. Group, ExCELLS)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[3P-359_1SP-07-02] Application of chromosome engineering technology (9):Identification of novel housekeeping gene promoter activity enhancing sequences

^○Rio Suzuki¹, Natsumi Miyazaki¹, Arisa Fujihara¹, Chihiro Onodera¹, Narumi Uno¹, Kazuki Yasuhiro^2,3, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 2.CERC, Tottori Univ., 3.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ.)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[3P-360_1SP-06-01] Application of chromosome engineering technology (9) Development of a bottom-up approach to generate mice that retain the BAC-linked HLA gene region

^○Reika Yuno¹, Nanami Kishima¹, Takashi Moriwaki¹, Narumi Uno², Kanako Kazuki³, Wakana Nakagawa¹, Teruhiko Suzuki⁴, Kazuma Tomizuka², Yasuhiro Kazuki^1,3,5,6 (1.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ.,, 2.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci.,, 3.CERC, Tottori Univ.,, 4.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci.,, 5.Chr. Eng. Group, ExCELLS.,, 6.Sch. of Life Sci., Facul. of Med., Tottori Univ.,)

The 46th Annual Meeting of the Molecular Biology Society of Japan

[3P-361_2SP-05-02] Application of chromosome engineering technology (11): Development of a top-down approach to generate mice that retain minimalized HLA gene region derived from native human chromosome

^○Nanami Kishima¹, Hitomaru Miyamoto¹, Satoshi Abe², Kyotaro Yamazaki³, Akane Okada⁴, Wakana Nakagawa¹, Reika Yuno¹, Takashi Moriwaki^2,4, Narumi Uno⁵, Kazuma Tomizuka⁵, Yasuhiro Kazuki^1,2,3,4 (1.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ.,, 2.Sch. of Life Sci., Facul. of Med., Tottori Univ.,, 3.Chr. Eng. Group, ExCELLS：ExCELLS.,, 4.CERC, Tottori Univ., 5.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci.)

The 46th Annual Meeting of the Molecular Biology Society of Japan