[P3] Abiotic Stress for Crop Production

[P3-27] Breeding for Submergence-Tolerant Rice by Marker Assisted Backcross

^○Yu-Chien Tseng¹, Yu-Chia Hsu¹, Yu-Chin Chang², Yong-Pei Wu² (1.Agronomy Department, National Chiayi University, Taiwan, 2.Agronomy Department, Chiayi Agricultural Experiment Station, Taiwan Agricultural Research Institute, Taiwan)

Rice (Oryza sativa L.) is an important food crop in the world. Due the climate change, to develop a rice variety which can tolerate the abiotic stress becomes a critical research topic. On this study, submergence-tolerant indica variety, IR96321-315-240 was used as donor parent. DT3 is the recurrent parent, which is drought tolerant and show good yield, eating quality and agronomic traits as elite Taiwanese variety, Taiken 9 (TK9). Marker-assisted selection (MAS) was applied in backcross breeding method. For foreground selection, there were two submergence-tolerant markers, Sub1A and SubAB1, utilized on BC₂F₁, BC₃F₁ and BC₃F₂ generations to select submergence-tolerant gene, Sub1A. Also, the flooding experiment in the field was applied in BC₃F₂ generation and the surviving plants then used for foreground and background selection.The results showed the similarity between surviving plants and recurrent parent was 92.87%. There are 100 plants evaluated for agronomic traits, yield and eating quality from BC₃F₃ generations. Eleven plants were selected and three of them had higher yield than DT3; three of them had better eating quality than DT3. By MAS, the submergence-tolerant trait has been successfully delivered to a drought tolerant, high yield and quality rice variety.