[P3] Abiotic Stress for Crop Production

[P3-33] Comparative Transcriptome Analysis in Sorghum (Sorghum bicolor L.) Leaves during Vegetative Stage under Waterlogging Stress

^○Ku Hyun Kwon¹, Sang-Heon Choi¹, Ju-Young Choi¹, Soo-Jeong Kwon¹, Hyen-Chung Chun², Dong-Gyu Lee¹, Seong-Hyun Yu¹, Tae-Woong Yun¹, Sun Hee Woo¹ (1.Department of Crop Science, Chungbuk National University, Korea, 2.National Institute of Crop Science, RDA, Korea)

Waterlogging stress induces dramatical alterations to sorghum growth and development. However, little information on the waterlogging tolerance and associated mechanisms of sorghum is known. Presently, several morpho-physiological indexes and transcriptome profiling under waterlogging stress were investigated during the 3- and 5-leaf stages in sorghum. Growth characteristics of sorghum showed significant differences in the plant height, stem length, and SPAD values under waterlogging stress compared to untreated seedlings. The functional annotation revealed that the top GO enriched DEGs, based on biological process, were involved in transcription and secondary metabolite biosynthetic processes at 3-leaf stages while the flavonoid biosynthetic process, flavonoid glucuronidation, secondary metabolite was counted as the top GO enriched DEGs in 5-leaf. In KEGG pathway enrichment based on RNA-Seq data, the top GO enriched DEGs are involved in phenylpropanoid biosynthesis, plant hormone signal transduction, and glutathione metabolism in 3-leaf stage. However, in 5-leaf stage, the top GO enriched DEGs are involved in plant hormone signal transduction, photosynthesis, glutathione metabolism. Under waterlogging stress, the plant hormone signal transduction pathways tended to be down-regulated towards all hormone signaling pathways except SA at 3-leaf stage. The over-expression of GST enzyme-related pathways in both the 3-leaf and 5-leaf stage may provide a deeper understanding of the mechanism underlying the response to waterlogging and guidance for the breeding of sorghum.