[2D01-05] Radiation Medicine and Biological Influence

[2D03] Fundamental Research on DNA Irradiation Analysis with He Ion Microbeam for α-ray Therapy

*Masaya Sakai¹, Tokihiro Ikeda², Atsushi Shibata³, Kento Takemoto¹, Mitsuru Uesaka¹ (1. Univ.Tokyo, 2. RIKEN, 3. Gunma Univ.)

Keywords:He ion microbeam, Glass capillary , Cell irradiation, DNA repair, α-ray therapy

Radiation therapy is one of the treatment methods for cancer, and particle therapy can minimize radiation exposure to normal tissues compared to photon radiation in vivo and can pinpoint cancerous lesions. However, the biological effects of heavy ion beams are often unknown. The purpose of this study is to measure radiation exposure and DNA damage to individual cells using glass capillary micro beam, which enables irradiation to individual cells and to analyze fate decisions. In this study, we will conduct visualization experiments of DNA damage with He ²⁺ glass capillary microbeam irradiation using a Pelletron accelerator. We visualize differences in macroscopic DNA damage distribution with microbeams having different LET using Mylar film, and perform time-lapse observation of the distribution of repair protein XRCC1. Based on this experiment, we will discuss the exact LET and DNA damage levels for DNA damaged individual cells and the subsequent cell fate determination.