[2N17-21] Medical Application/Applied Measurement

[2N18] Mechanistic Study Comparing Radiolabeled and Fluorescent Glucose Uptakes by Cancer Cells for In Vivo Bioimaging

*Keiichiro Kushiro¹, Mariko Kobayashi¹, Akira Sugiyama¹, Kenji Shimazoe¹, Hiroyuki Takahashi¹ (1. The University of Tokyo)

Keywords:Bioimaging, Cancer, PET, FDG

Although both PET and fluorescence imaging based on glucose analogs have been used to study tumors in vivo, there have been some inconsistencies between them due to the lack of the mechanistic understandings underlying the uptake of the gluocose-based probes. In this study, quantitative analyses of the localization and kinetics of glucose uptake when labeled with either radioactive isotopes or fluorecent molecules were performed. After incubating ovarian cancer cells with either FDG or RediJect 2-DG at different temperatures and culture conditions, the cells were separated into the membrane and cytosolic components via ultracentrifugation, and the fluorescence or radiation signals were measured for each component. Understanding the mechanisms underlying this uptake phenomena will lead to a more accurate tumor bioimaging.