[1G01-05] Beam Application

[1G05] Analysis of the injured microbial cells appeared by irradiation of Bacillus subtilis spores based on their germination and outgrowth deteorization as a probe

*Masakazu Furuta¹, Akira Futemma¹, Hiroki Den¹, Jin Sakamoto², Tetsuaki Tsuchido² (1. Department of quantum radiation sciences, College of Engineering, Graduate school of Osaka Prefecture University, 2. Research Center of Microorganism Control, Research Center for the 21st Century, Osaka Prefecture University)

Keywords:Bacillus subtilis, bacterial spores, irradiation, heating, decontamination/sterilization, injured microbes

As bacterial spores show high registance against various cell-killing stresses, efficient decontamination methods are required. In this study, heat- or gamma-ray-treated Bacillus subtilis spores were analyzed for distinguish the cell-killing mechanism based on the change of germinaion and outgrowth pattern during the recovery of the cells.