[1F01-05] Medical and Biological Application 1

[1F03] Fundamental Research on DNA Irradiation Analysis with He Ion Microbeam for α-ray Therapy

*Masaya Sakai¹, Tokihiro Ikeda², Atsushi Shibata³, Masako Izumi², Kento Takemoto¹, Mitsuru Uesaka¹ (1. Univ. of Tokyo, 2. RIKEN, 3. Gunma Univ.)

Keywords:He ion microbeam, Glass capillary, Cell irradiation, DNA repair, α-ray therapy

Although α-ray treatment has the merit of being able to irradiate the carcinomatous lesion with a pinpoint, the biological effects of α-rays at the cellular level have not yet been clarified in many cases. The purpose of this study is to analyze three-dimensionally the amount of DNA damage of individual cells with a glass capillary microbeam which can irradiate individual cells. This time, we conduct DNA damage visualization experiments on RPE cells with 4.5 MeV He²⁺ microbeam using a Peletron accelerator. Furthermore, we produce a plurality of samples by changing the time for fixing the cells after irradiation, and observe the repair protein distribution in a time-sharing manner. Based on this experiment, we examine the amount of DNA damage and the repair process for individual DNA damaged cells.