11:15 AM - 11:30 AM
[1N04] Radiation Protection by a Tiny amount of Additive:
Yields Measurements of Radiation Damage to DNA without Protective Agents
Keywords:DNA damage, radiation protective agent, chemical recovery, radical scavenger, radiation therapy
In radiation therapy of cancer, using protective agent is conceivable to minimize the radiation effects on normal cells. Since the concentration of protective agent in cell is low, radiation protection effect of trace additive is important. Tris-EDTA, a solute of TE buffer used in previous study has high protection effect. To research protection effect of trace additive, it needs investigation of the addition effect under the condition that TE is removed. In this study, we established the method elucidating the initial process of radiation protection of trace additive.
TE buffer for preserving plasmid DNA was replaced with phosphate buffer. DNA samples were irradiated and the damage yields were determined by electrophoresis. Finally, we studied the protection effect of additive.
TE buffer for preserving plasmid DNA was replaced with phosphate buffer. DNA samples were irradiated and the damage yields were determined by electrophoresis. Finally, we studied the protection effect of additive.