Academic Events » Symposium

3SAA Toward a standardization of luminescence, fluorescence measurements and light microscopy

Fri. Sep 30, 2022 9:00 AM - 11:30 AM Room A (Hakodate Arena Budokan A)

Organizers:Akira Sasaki(AIST),Yoshihiro Ohmiya(AIST)

The quantitative aspect of luminescence, fluorescence measurement and light microscopy is becoming significant. The challenge now lies in improving the accuracy and precision of the data obtained from such measurements. Standardization is the way to achieve precise, reproducible and inter-comparable measurement. Improving these will facilitate the comparison of results between different instruments/institutions and therefore ensure the reproducibility of results. In this symposium, recent standardization effort in the world (e.g. ISO) will be introduced in addition of leading edge researches of the related field.

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9:00 AM - 9:02 AM

[3SAA-00] Opening Remarks

9:02 AM - 9:15 AM

[3SAA-1] Toward traceable quantitative fluorescence microscopy - Benchmarking microscope using FCS technique -

〇Akira Sasaki¹ (1.BMRI, AIST)

9:15 AM - 9:45 AM

[3SAA-2] Supporting cellular analysis by quantitative imaging with standards and reference materials

〇Michael Halter¹, Ed Kwee¹, Alexander Peterson¹, John T Elliott¹ (1.National Institute of Standards and Technology (NIST), USA)

9:45 AM - 10:03 AM

[3SAA-3] Towards improvement of the reliability of cell morphometry for analysis and manufacturing of cells - Role of ISO standardization

〇Yoshiko Nomi¹ (1.Chiyoda Corporation)

10:03 AM - 10:14 AM

[3SAA-4(1Pos289)] Morphological Analysis of Hydrogel Induced Cancer Stem Cells in Synovial Sarcoma Model Cells

〇Zannatul Ferdous¹, Masumi Tsuda^1,3,4, Jean-Emmanuel Clément³, Jian Ping Gong^1,3,6, Shinya Tanaka^3,4,6, Tamiki Komatsuzaki^2,3,5, Koji Tabata² (1.Graduate School of Life Science, Hokkaido University, 2.Research Center of Mathematics for Social Creativity, Research Institute for Electronic Science, Hokkaido University, Sapporo, Japan, 3.Institute for Chemical Reaction Design and Discovery (WPI-ICReDD), Hokkaido University, Sapporo, Japan, 4.Department of Cancer Pathology, Hokkaido University Faculty of Medicine, Sapporo, 5.Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, Japan, 6.Global Station for Soft Matter, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan)

10:14 AM - 10:44 AM

[3SAA-5] Quantification of receptor clustering and activation at the cell surface using correlation and lifetime-based methods

〇Andrew Harry Albert Clayton¹ (1.Cell Biophysics, Optical Sciences Centre, Swinburne University of Technology, Hawthorn, Australia)

10:44 AM - 10:55 AM

[3SAA-6(1Pos288)] Size determination of cytoplasmic condensates of optineurin using spatial image correlation spectroscopy (SICS)

〇Yuta Hamada¹, Masataka Kinjo², Akira Kitamura² (1.Grad. Sch. Sci. of Life Sci., Hokkaido Univ., 2.Fac. of Adv. Life Sci., Hokkaido Univ)

10:55 AM - 11:13 AM

[3SAA-7] Absolute light measurement for the investigation of bioluminescence quantum yield and standardization of bioanalysis instruments

〇Kazuki Niwa¹ (1.National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and technology (AIST))

11:13 AM - 11:26 AM

[3SAA-8] Quantitative bioluminescence imaging in vitro and ex vivo

〇Yoshihiro OHMIYA^1,2 (1.AIST, 2.Osaka Institute of technology)

11:26 AM - 11:30 AM

Session information