Academic Events » Symposium

3SAA Functional elucidation of liquid-liquid phase separation (LLPS) based on structural biology approach

Thu. Nov 16, 2023 9:00 AM - 11:30 AM Room A (Exhibition Room 211 (Bldg. 2, 1F))

Organizers: Nishida Noritaka (Chiba Univ.)、Ikeya Teppei (Tokyo Metro. Univ.)

Liquid-liquid phase separation (LLPS) is driven by the dynamic assembly of diverse protein and RNA molecules in the cells. In order to understand how such droplets, which are seemingly disordered structures, regulate various physiological functions, it is necessary to quantitatively measure the behavior of LLPS at the atomic and molecular levels. In this symposium, we would like to introduce recent studies of researchers who are aiming to elucidate the principles of LLPS formation and the regulation of intracellular functions by structural biology approaches such as NMR and computational science, as well as chemical biology.

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9:00 AM - 9:25 AM

[3SAA-1] Analysis of the mechanism underlying multivalent interactions between GRB2 and SOS1 and their LLPS using solution NMR

〇Teppei Ikeya¹, Mao Oide², Weitong Ren², Keita Tateno¹, Takashi Ando¹, Haruka Sugasawa¹, Yuji Sugita², Yutaka Ito¹ (1.Grad. Sch. Sci., Tokyo Metropolitan Univ., 2.RIKEN CBR)

9:25 AM - 9:50 AM

[3SAA-2] Mapping the per-residue surface electrostatic potential of CAPRIN1 along its phase-separation trajectory

〇Yuki Toyama^1,2,3, Atul Rangadurai^1,2,3,4, Julie Forman-Kay^2,4, Lewis Kay^1,2,3,4 (1.Department of Molecular Genetics, University of Toronto, 2.Department of Biochemistry, University of Toronto, 3.Department of Chemistry, University of Toronto, 4.Hospital for Sick Children, Program in Molecular Medicine)

9:50 AM - 10:15 AM

[3SAA-3] Preparation and analysis of aggregates formed by the tumor suppressor protein p53

〇Emi Hibino¹, Reiji Hijikata¹, Takeshi Tenno^1,2, Hidekazu Hiroaki^1,2 (1.Grad. Sch. Pharm. Sci, Nagoya Univ., 2.BeCellBar)

10:15 AM - 10:40 AM

[3SAA-4] Liquid-liquid phase separation of protein assemblies studied by molecular simulations

〇Shoji Takada, Azuki Mizutani, Risa Yamada, Yutaka Murata (1.Grad. Sch. Sci. Kyoto Univ)

10:40 AM - 11:05 AM

[3SAA-5] Designer protein condensates for cell signal manipulation

〇Shinya Tsukiji (1.Grad. Sch. Eng., Nagoya Inst. Technol.)

11:05 AM - 11:30 AM

[3SAA-6] In-cell NMR Observation of Liquid-Liquid Phase Separation of FUS

〇Noritaka Nishida (1.Grad. Sch. Pharm. Sci., Chiba Univ.)