[5-1015-D] Other Categories (2)

[5-1015-D-01] Screening and Enzyme Activity of Cellulose-Producing Bacteria Isolated from Kemiri Sunan (Reutealis trisperma (Blanco) Airy Shaw) and Empty Fruit Bunches of Palm Oil

*Tri Yuliana¹, Efri Mardawati¹, Souvia Rahimah¹, Emilda Ayu Febrianty¹, Agus Try Hartono¹ (1. Univ. Padjadjaran, Indonesia(Indonesia))

キーワード：cellulose, Reutealis trisperm, Palm bunches, clear zone, enzyme activity

Biocatalyst technology is needed for the industry to improve performance of production. Cellulase enzymes has an important role in biocatalyst technology, especially in pulp industry. Cellulase is produced by certain types of microbes. The selection of cellulase-producing bacteria from Trisperma shell and empty fruit bunches from oil palm were carried out in order to produce cellulase which can be used for the pulp industry. Effectiveness test of cellulase-producing bacteria from Trisperma shell and palm bunches were also carried out using the liquid phase fermentation method. The result shows isolat K2 gave the widest clear zone with a value of 77.19% ± 0.00835 in BSM-CMC-CR media. OD value was calculated within 8 hours, 24 hours, 32 hours, and 48 hours in NB media. The result shows at 32 hours, the K3 isolate gave the highest absorbance with the value of 0.9163. Test of enzyme activity shown the K3 isolate had a highest enzyme activity with its value of 43.2 x 10-5 U/mL at 48 hours. The result of gram negative bacteria staining was assumed that the bacteria was Pseudomonas sp.