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[6-1130-P-01] Primary Prebiotic Properties of Ethanolic Sugar Extract from Groundnut Seeds
Keywords:Groundnut, Arachis hypogaea, Raffinose Family Oligosaccharides, Prebiotic, Probiotic, Functional Food
Raffinose family oligosaccharides (RFOs) have been accepted as an effective prebiotic substance. They can be generally found in various leguminous seeds. Thus, legume seeds can be considered as promising sources of prebiotic ingredient for development of functional foods. The aims of this work were analysis of RFOs composition in local groundnut (Arachis hypogaea L.) of Thailand and primary investigation for their prebiotic potential. In this study, low molecular weight sugars (LMWSs) including RFOs were extracted from seeds of three local groundnut cultivars in Thailand, i.e. Tainan 9, Khonkean 5 and Khonkean 6, using 50% (v/v) ethanol. LMWSs were qualified and quantified by HPLC apparatus and subsequently investigated for their capacity in growth stimulation of some enteric bacteria. The results showed that these cultivars contained LMWSs approximately 28-40 mg/g dry seed and the average size of sugars in term of degree of polymerization (DP) ranged between 2 and 7. These seeds contained low amount of raffinose and verbascose, while high amount of stachyose was found at 3.9-11.7 mg/g dry seed. Growth of probiotic Lactobacillus acidophilus TISTR1338, L. plantarum TISTR541 and L. lactis TISTR1464 were stimulated significantly in basal media containing groundnut LMWSs (p<0.05), while growth of Salmonella enterica serovar Typhimurium TISTR292 and Escherichia coli were not stimulated. Interestingly, growth of S. Typhimurium and E. coli were suppressed when was co-cultured with those Lactobacillus sp. in basal media contained groundnut LMWSs as a carbon source. Thus, it might be concluded that ethanolic sugar extracted from seeds of Tainan 9, Khonkean 5 and Khonkean 6 exhibited the primary properties to be accepted as prebiotic substance.