[6-1130-P] Functional/Wellness Foods & Nutrition (6th)

[6-1130-P-03] Evaluation of Total Anthocyanins and Antioxidant Activity of Thai Rice Cultivars for Phenotypic Selection in Rice Breeding

*Chotipa Sakulsingharoj¹, Lalita Na Rachasima¹, Anongnad Richinda¹, Pairote Wongputtisin², Rungthip Kawaree², Saengtong Pongjaroenkit¹, Varaporn Sangtong¹ (1. Program in Genetics, Faculty of Science, Maejo University, Chiang Mai, Thailand(Thailand), 2. Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai, Thailand(Thailand))

キーワード：Extraction, Anthocyanins, Antioxidant activity, Thai black rice

Black rice has been gained increasing interest for consumers and rice breeders due to its high nutritional values of anthocyanin contents and antioxidative properties. The objective of this study was to determine the optimal solvents for anthocyanin extraction and quantification of antioxidant activity for selection of Thai rice cultivars with high anthocyanins and antioxidant activity to be used in rice breeding program. The dehulled mature seeds of Thai black rice cv. Hom Nin were extracted by different solvent types and the extracts were evaluated for total anthocyanin contents and antioxidant activity by spectrophotometry and DPPH assay, respectively. The results demonstrated that the extract with 1% HCl in 80% methanol gave the highest total anthocyanins and antioxidant activity. This solvent was subsequently used for extraction of seeds from eight rice cultivars, which consisted of four non-pigmented (white) and four black rice cultivars. It was found that the extracts from black rice cultivars showed no significantly different levels of antioxidant activity, possibly due to interference by hydrochloric acid in DPPH assay. Therefore, 80% methanol was used for anthocyanin extraction of rice cultivars. The results showed that antioxidant activity had positive correlation with amount of total anthocyanin contents and phenotypic traits of pericarp colors. In this study, Thai black rice cv. Mali Dum (MLD) gave the highest total anthocyanin contents and antioxidant activity which were correlated with coloration of extracted sample and pericarp color. Our study suggested that MLD would be a good source of high anthocyanins and antioxidant activity for use as parental line in rice breeding program for improvement of rice with health promoting properties. Moreover, advanced breeding lines with high anthocyanin contents and antioxidant activity could be identified by methanolic extraction method followed by spectrophotometric measurements and DPPH assay.