[P5-06] Oral and intestinal bacterial flora in centenarians and octogenarians with low functional independence in nursing homes
【抄録（Abstract）】
Aim: The purpose of this study is to analyze the oral and intestinal bacterial flora of low-functioning octogenarians and centenarians.
Methods: Saliva and fecal samples were collected from nursing home residents who all received meals from the same catering company. Ten octogenarians (84–89 years old) and 8 centenarians (100–103 years old) with Barthel Index (BI) scores < 60 were included. A dentist counted the number of teeth, and evaluated the denture and nutritional status by Mini Nutritional Assessment Short Form. Bacterial DNA was extracted and multiplex 16S rRNA (V3–V4) sequencing was performed using the Illumina MiSeq platform. Composition and diversity were analyzed by the Mann-Whitney U test and weighted PCoA.
Results: The oral microbial diversity of centenarians was slightly higher than that of octogenarians but fecal samples displayed an opposite pattern; however, the differences were not significant. Oral flora composition differed between the two groups, likely due to the lower number of teeth in centenarians. Despite having a similar number of teeth, there is a significant difference in the Mini Nutritional Assessment Short Form between healthy and unhealthy Octogenarians(P<0.001), but this difference is not reflected compared with centenarians(P=0.596) which may be caused by almost all centenarians used dentures that helped restore posterior occlusion.
Conclusions: Age and physical health can affect the changes in the oral and intestinal flora of the elderly. However, restoration of masticatory function by dentures may help maintain nutritional status, regardless of age.
The author’s declaration: No conflict of interest (COI), in any way. Matsumoto Dental University Ethics Committee Approval Number 0302.
Aim: The purpose of this study is to analyze the oral and intestinal bacterial flora of low-functioning octogenarians and centenarians.
Methods: Saliva and fecal samples were collected from nursing home residents who all received meals from the same catering company. Ten octogenarians (84–89 years old) and 8 centenarians (100–103 years old) with Barthel Index (BI) scores < 60 were included. A dentist counted the number of teeth, and evaluated the denture and nutritional status by Mini Nutritional Assessment Short Form. Bacterial DNA was extracted and multiplex 16S rRNA (V3–V4) sequencing was performed using the Illumina MiSeq platform. Composition and diversity were analyzed by the Mann-Whitney U test and weighted PCoA.
Results: The oral microbial diversity of centenarians was slightly higher than that of octogenarians but fecal samples displayed an opposite pattern; however, the differences were not significant. Oral flora composition differed between the two groups, likely due to the lower number of teeth in centenarians. Despite having a similar number of teeth, there is a significant difference in the Mini Nutritional Assessment Short Form between healthy and unhealthy Octogenarians(P<0.001), but this difference is not reflected compared with centenarians(P=0.596) which may be caused by almost all centenarians used dentures that helped restore posterior occlusion.
Conclusions: Age and physical health can affect the changes in the oral and intestinal flora of the elderly. However, restoration of masticatory function by dentures may help maintain nutritional status, regardless of age.
The author’s declaration: No conflict of interest (COI), in any way. Matsumoto Dental University Ethics Committee Approval Number 0302.