3:10 PM - 3:20 PM
[C-10] High-resolution detection of oral microbial transmission from mother to infant
Keywords:Oral microbiota、Mother-infant transmission、Long-read sequencing
Background: Acquisition of maternal oral microbes is considered to play an important role in the development of infant oral microbiota. In this study, we evaluated oral microbial transmission from mother to infant and identified the factors influencing transmission.
Methods: We collected tongue swab samples from 448 mother-infant pairs at 4-month checkup. The bacterial composition of each sample was determined using PacBio single-molecule long-read sequencing of the full-length 16S rRNA gene and the amplicon sequence variant (ASV) approach. These approaches enable high-resolution validation for the mother-infant transmission of oral bacteria based on the ASV sharing (i.e., identical match of full-length 16S rRNA gene).
Results: Although the infant oral microbiota was distinctly different from the mother oral microbiota, at least 1 ASV was shared with biological mother in 91.1% of infants. They accounted for 9.7% (0-99.3%) of relative abundance in each infant microbiota (median [range]). This shared abundance was strongly associated with the feeding method of infants rather than their delivery mode or antibiotic exposure, and formula- and mixed-fed infants had higher shared abundance than exclusively breastfed infants.
Conclusions: Our study presents strain-level evidence for extensive mother-to-infant transmission of oral bacteria and suggests that breastfeeding may delay early maturation of oral microbiota in infants.
Methods: We collected tongue swab samples from 448 mother-infant pairs at 4-month checkup. The bacterial composition of each sample was determined using PacBio single-molecule long-read sequencing of the full-length 16S rRNA gene and the amplicon sequence variant (ASV) approach. These approaches enable high-resolution validation for the mother-infant transmission of oral bacteria based on the ASV sharing (i.e., identical match of full-length 16S rRNA gene).
Results: Although the infant oral microbiota was distinctly different from the mother oral microbiota, at least 1 ASV was shared with biological mother in 91.1% of infants. They accounted for 9.7% (0-99.3%) of relative abundance in each infant microbiota (median [range]). This shared abundance was strongly associated with the feeding method of infants rather than their delivery mode or antibiotic exposure, and formula- and mixed-fed infants had higher shared abundance than exclusively breastfed infants.
Conclusions: Our study presents strain-level evidence for extensive mother-to-infant transmission of oral bacteria and suggests that breastfeeding may delay early maturation of oral microbiota in infants.