1:00 PM - 1:30 PM
[JK-01] Three-dimensional topography of the neurons and neuroprotection by M2 macrophages in the trigeminal ganglion.
Keywords:3D topography、macrophage、trigeminal ganglion
The cell bodies of trigeminal ganglion neurons are surrounded by satellite cells, and there is a close interaction between neurons and satellite cells, as well as between adjacent satellite cells, through gap junctions and neuropeptide and ATP signaling. This cytoarchitecture is thought to be one of the mechanisms that give rise to allodynia and iatrogenic pain in the orofacial regions. A precise three-dimensional somatotopy of trigeminal ganglion cells was revealed by a combination of retrograde labeling with fast blue and tissue clearing (modified 3DISCO). In the trigeminal ganglion, neurons innervating the first, second, and third divisions were distributed in distinct areas, but at the boundaries, the distribution of cell bodies overlapped. The distribution of neurons innervating the head skin, upper eyelid, cornea, and dura mater of the first division overlapped to such a high degree that they were able to interact with each other. Furthermore, the distribution of neurons innervating the third division, the lingual mucosa, masseter muscle, temporalis muscle, and molar pulp, was also highly overlapping. These results suggest that ganglion cells innervating these regions may co-activate each other via satellite cells and cause ectopic pain.
Neuronal function in the trigeminal ganglion is regulated by macrophage-like cells and satellite cells. Normally, the cell bodies of ganglion neurons are surrounded by satellite cells and are not in direct contact with macrophages. However, we found that when the trigeminal nerve is injured, the tissue-protective M2 macrophages resident in the trigeminal ganglion are activated and come into direct contact with the damaged neuronal cell bodies. This direct interaction between ganglion neurons, satellite cells, and macrophages within the trigeminal ganglion was shown to act as a protective system for damaged neurons.
Neuronal function in the trigeminal ganglion is regulated by macrophage-like cells and satellite cells. Normally, the cell bodies of ganglion neurons are surrounded by satellite cells and are not in direct contact with macrophages. However, we found that when the trigeminal nerve is injured, the tissue-protective M2 macrophages resident in the trigeminal ganglion are activated and come into direct contact with the damaged neuronal cell bodies. This direct interaction between ganglion neurons, satellite cells, and macrophages within the trigeminal ganglion was shown to act as a protective system for damaged neurons.