Poster session

[P1-2-30] Role of Streptococcus mutans rhamnose-glucose polysaccharide (RGP) in colonization in mouse organs

〇Taiki Ando¹, Tomomi Hashizume-Takizawa², Kazuhiro Aoki¹, Hidenobu Senpuku² (1. Dept Basic Oral Health Eng, Tokyo Med Dent Univ Grad Sch Med Dent Sci, 2. Dept Micribiol Immunol, Nihon Univ Sch Dent at Matsudo)

Keywords:Streptococcus mutans、RGP、定着

Streptococcus mutans, a cariogenic bacterium is known to be related to systemic disorders including infective endocarditis. It has been reported that rhamnose-glucose polysaccharides (RGP) which are constituents of cell wall of S. mutans seem to play an important role in induction of infective endocarditis. Thus, in this study, we explored the role of RGP in S. mutans localization in murine systemic tissues after intravenous administration by employing luciferase(renG)-expressing RGP gene deletion mutant of S. mutansUA159 (SMU833^-). We intravenously administered recombinant S. mutans to mice and bioluminescent signals in murine organs were measured. Our results showed that bioluminescent signals were detected from kidney and spleens which indicating localization of recombinant S. mutans at these tissues. Further, the signal intensity detected from kidney of mice injected with renG-expressing S. mutans SMU833^- were lower than those of mice given renG-expressing S. mutans UA159. These results suggested that the RGP may be involved in colonization of S. mutans in kidney of mice after intravenously administration.