[P1-3-12] The positive effects of leukocyte- and platelet-rich plasma (l-prp) on osseointegration after implant placement in mouse maxilla
Keywords:dental implants、platelet-rich plasma、osseintegration
Purpose: This study aimed to analyze the effect of leukocyte and platelet-rich plasma (L-PRP) on osseointegration in the bone-implant interface after immediate placement of implants in the maxilla of 4-week-old male ICR mice. Method: After venous blood was collected from the tail vein of 4-6-week-old male mice in a tube containing an anticoagulant (ACD), blood cells were counted using an automatic blood cell counting device. Following the right upper first molars of 4-week-old mice were extracted under deep anesthesia, implant cavities were prepared with a drill, filled with or without 1.5 uL of L-PRP (experimental or control groups [EG or CG]), and replaced with titanium implants. Perfusion fixation was performed at 3, 5, 7, 14 and 28 days after implantation. After decalcification, paraffin sections were analyzed using immunohistochemistry (Osteopontin [OPN] and Ki-67) and TRAP histochemistry. Results: The cell proliferation was significantly higher in the EG compared with the CG 3 days after operation. The OPN-positive perimeter and osseointegration rate (direct and indirect osteogenesis) in the EG tended to be higher than those in the CG at day 7, and the EG became a similar rate of cell proliferation with the CG. Conclusion: These results suggest that the L-PRP could positively affect proliferative activity around the implant in the early stages after implant placement to increase the deposition of OPN at the bone-implant interface and osseointegration.