[P1-3-33] The effect of impaired store-operated Ca2+ entry in the epithelial cells
Keywords:ストア作動性カルシウム流入、皮膚、バリア機能
[Purpose] Elucidating the role of store-operated Ca2+ entry (SOCE) in the epidermis by analyzing the mice with Stim1 and Stim2 gene ablation. [Materials & Methods] Eight-week-old male mice with epithelial tissue-specific deletion of Stim1 and Stim2 (Stim1/2K14Cre : cKO) and those control (Stim1/2fl/fl) were utilized. Keratinocytes (KC) were isolated from mice in both groups, and the protein expressions of Stim1/2 were compared by Western blotting method (WB). Histological examinations of the epidermis were performed by H-E staining and immunohistochemistry (IHC). The SOCE in KC was measured using the fluorescent dye Fura-2. The expression levels of epidermal differentiation and inflammatory markers were confirmed by quantitative PCR. Transepidermal water loss (TEWL) in the back skin of mice was measured. [Results & Conclusion] WB analysis indicated that the gene expressions of Stim1/2 were decreased in cKO mice. Although immunohistochemistry revealed that the signal intensities from Stim1/2 were decreased in cKO mice, no substantial changes were found in epidermis morphology. The gene expression levels of Filaggrin, Asprv1, Tslp, and Ccl17 were significantly elevated, as well as a significant increase in TEWL in cKO mice. These results suggest that the decreased SOCE is involved in the inflammatory response and barrier of the epidermis