Poster session

[P2-2-01] Sclerostin deficiency effectively promotes bone morphogenetic protein-2-induced ectopic bone formation

〇Masanori Koide¹, Kobayashi Yasuhiro¹, Teruhito Ymashita¹, Nobuyuki Udagawa^1,2 (1. Matsumoto Dent Univ, 2. Dept Oral Biochem, Matsumoto Dent Univ)

Keywords:スクレロスチン、BMP-2、異所性骨

The application of bone morphogenetic protein-2 (BMP-2) has been attempted for bone fractures and severe alveolar bone loss. BMP-2 reportedly induces sclerostin expression, an inhibitor of Wnt signals, that attenuates bone acquisition. However, the effect of sclerostin-deficiency on BMP-2-induced bone regeneration has not been fully elucidated. We investigated BMP-2-induced ectopic bones in Sost-knockout (KO) mice. rhBMP-2 were implanted into the thighs of C57BL/6 (WT) and Sost-KO male mice at 8 weeks of age. The BMP-2-induced ectopic bones in these mice were examined on days 14 and 28 after implantation. Micro-computed tomography analysis revealed that BMP-2-induced ectopic bones in Sost-KO mice showed a significant increased relative bone volume and bone mineral density compared with those in WT mice on days 14 and 28 after implantation. BMP-2-induced ectopic bones in Sost-KO mice showed an increased horizontal cross-sectional bone area on day 28 after implantation. Taken together, Sclerostin deficiency increased bone mineral density in BMP-2-induced ectopic bones.