[P3-2-26] Effects of Phellodendron bark extract on periodontal pathogenic bacteria in an in vitro oral microbiome model
Keywords:歯周病、オウバクエキス、細菌叢
Periodontal disease is triggered by dysbiosis in the oral microbiome. It is important to reduce the relative abundance of periodontal pathogenic bacteria in the oral microbiome to prevent periodontal disease. Phellodendron bark extract (PBE) is a crude drug ingredient with anti-inflammatory effects. Although PBE exerts antibacterial effects on periodontal pathogenic bacteria, such as Porphyromonas gingivalis, its effects on the oral microbiome as a whole remain unknown. We therefore aimed to clarify the potential of PBE in regulating the relative abundance of periodontal pathogenic bacteria in the oral microbiome at a low level. Saliva was collected from 20 subjects with their prior consent. Each saliva and Porphyromonas gingivalis suspension was added to a medium with or without PBE and incubated under anaerobic conditions for 24 hours. After cultivation, we determined the total bacterial concentration using quantitative polymerase chain reaction (qPCR) analysis and the bacterial composition using 16S ribosomal RNA gene sequencing. qPCR analysis showed that the total bacterial concentration was reduced by treatment with PBE. Bacterial 16S ribosomal RNA gene sequencing revealed that treatment with PBE significantly reduced the relative abundance of periodontal pathogenic bacteria, such as red complex and orange complex bacteria. These results suggest that PBE could reduce the relative abundance of periodontal pathogenic bacteria in the oral microbiome. PBE could help to prevent periodontal disease through its ability to regulate the composition of the oral microbiome and its anti-inflammatory effects.