[P3-2-35] Methylglyoxal suppresses the growth of oral bacteria
Keywords:メチルグリオキサール、Porphyromonas gingivalis、Streptococcus mutans
[Purpose] Methylglyoxal (MG) is a ketoaldehyde with a chemical formula of CH3COCHO and is produced in vivo as a by-product of glycolysis. In recent years, it has been noted that MG contained in food exhibits bactericidal activity, but the effect of MG on the growth of oral bacteria has not been fully elucidated. Therefore, we analyzed the effect of MG on the growth of oral bacteria. [Materials and methods] Porphyromonas gingivalis (P. g.) and Streptococcus mutans (S. m.) were used as oral bacteria. Bacteria suspended in Brain Heart Infusion medium containing 1% Yeast Extract were seeded in a 96-well plate, MG was added at various concentrations, and after anaerobic culture at 37°C for 24 hours, absorbance was measured at a wavelength of 620 nm. Hemin, menadione, and cysteine were added to the culture system of P.g. In addition, after collecting the cells by centrifugation, they were washed with PBS, and statically cultured for 24 hours in an anaerobic environment at 37°C using sheep blood agar medium for P.g. and Mitis Salivarius agar medium for S.m. A minimum bactericidal concentration (MBC) was determined. [Results] The growth of P.g. cultured in liquid medium was inhibited in the presence of MG of 5mM or more. The MBC of P. g. determined on blood agar was also 5 mM. The growth of S. m. The MBC of S. m. determined on agar medium was 10 mM. [Conclusion] MG inhibited the growth of P. g at concentrations above 5 mM, and MBC against P. g was 5 mM. In addition, MG inhibited S. m. proliferation at concentrations of 2.5 mM or higher, and MBC against S. m. was 10 mM. These results indicated that MG inhibited the growth of oral bacteria.