[B-PT02_1AM2] Biocalcification and the Geochemistry of Proxies

[BPT02-09] 地球化学とゲノム生物学を繋げるためのコユビミドリイシサンゴを使った研究

*ベル 智子¹、横山 祐典¹、井口 亮²、鈴木 淳³ (1.東京大学大気海洋研究所、2.国立沖縄工業高等専門学校生物資源工学科、3.独立行政法人産業技術総合研究所)

キーワード：Acropora digitifera, Calcification, Gene, Skeletal elements

Coral's calcification mechanism has been receiving great attention in the fields of both geochemistry and biology. In geochemistry, high-resolution proxies using coral skeletal elements have been developed to reconstruct climate history (Gagan et al, 2012). In parallel, coral genomes have been sequenced progressively. However, trials that connect these two different fields of studies focusing on coral calcification have not been conducted yet. In this study, we focused on Acropora digitifera as the target species because enough genomic information is available (Shinzato, 2011) and its potential as geochemical proxies (Inoue, 2011). First, using ZoophyteBase, which has been recently developed as coral's proteome database (Dunlap et al, 2013), we investigated the genes that are potentially related to metabolism using inorganic minerals in seawater and analyzed their gene components and the correlations with seawater chemistry. Second, using next-generation sequencing, we are currently comparing Acropora digitefera's gene expression between fast and slow calcification lineages of this species. In addition, coral skeletal elements of these materials have been analyzed by ICP-AES. In this presentation, we report the progress of these analyses focusing on calcification related genes and skeletal elements. References: [1] Dunlap et al, 2013.BMC Genomics. DOI: 10.1029/2011PA002215 [2] Gagan, et al, 2012. Paleoceanography. DOI: 10.1029/2011PA002215 [3] Inoue et al, 2011. Geophysical Research Letters. DOI: 10.1029/2011GL047786 [4] Shinzato et al, 2011. Nature. DOI:10.1038/nature10249