Japan Geoscience Union Meeting 2014

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International Session (Oral)

Symbol B (Biogeosciences) » B-PT Paleontology

[B-PT02_1AM2] Biocalcification and the Geochemistry of Proxies

Thu. May 1, 2014 11:00 AM - 12:45 PM 421 (4F)

Convener:*Takashi Toyofuku(Institute of Biogeosciences (BioGeos), Japan Agency for Marine-Earth Science and Technology), Hiroshi Kitazato(Institute of Biogeosciences (BioGeos),Japan Agency for Marine-Earth Science and Technology (JAMSTEC)), Jelle Bijma(Alfred-Wegener-Institut fur Polar- und Meeresforschung), Chair:Takashi Toyofuku(Institute of Biogeosciences (BioGeos), Japan Agency for Marine-Earth Science and Technology)

11:45 AM - 12:00 PM

[BPT02-10] Comprehensive identification of shell matrix proteins in brachiopods

*Yukinobu ISOWA1, Isao SARASHINA2, Kenshiro OSHIMA3, Keiji KITO4, Masahira HATTORI3, Kazuyoshi ENDO1 (1.Graduate School of Science, Univ. Tokyo, 2.University Museum, Univ. Tokyo, 3.Graduate School of Frontier Sciences, Univ. Tokyo, 4.School of Agriculture, Meiji Univ.)

Brachiopods are marine invertebrates that appeared in the Cambrian, and they have two shells like bivalves composed of calcium carbonate or calcium phosphate. Shells contain organic matrix, which have important roles in the biomineralization processes. Recently, many shell matrix proteins in molluscs have been identified, and their roles in shell formation have been discussed. On the other hand, shell matrix proteins in brachiopods have not been identified, except for partial amino acid sequences of a chromoprotein, named ICP-1. In this study, we performed comprehensive identification of shell matrix proteins of the brachiopod Laqueus rubellus using proteomics combined with transcriptomics. As a result, we identified a total of 18 shell matrix proteins. BlastP search showed that these proteins have no homologues in skeletal proteins identified from other phylum, suggesting that brachiopod and mollusc shells are different in origin.