Japan Geoscience Union Meeting 2014

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Oral

Symbol M (Multidisciplinary and Interdisciplinary) » M-IS Intersection

[M-IS30_28PM1] Paleoclimatology and paleoceanography

Mon. Apr 28, 2014 2:15 PM - 4:00 PM 501 (5F)

Convener:*Kazuyoshi Yamada(School of Human Sciences, Waseda University), Minoru Ikehara(Center for Advanced Marine Core Research, Kochi University), Tomohisa Irino(Faculty of Environmental Earth Science, Hokkaido University), Yusuke Okazaki(Department of Earth and Planetary Sciences, Graduate School of Science, Kyushu University), Ikuko Kitaba(Kobe University Research Center For Inland Seas), Akihisa Kitamura(Institute of Geosciences, Faculty of Science, Shizuoka University), Masaki Sano(Research Institute for Humanity and Nature), Ryuji Tada(Department of Earth and Planetary Science, Graduate School of Science, The Univeristy of Tokyo), Masakazu Yoshimori(Atmosphere and Ocean Research Institute, University of Tokyo), Chair:Tomohisa Irino(Faculty of Environmental Earth Science, Hokkaido University)

2:30 PM - 2:45 PM

[MIS30-11] Identification of single pollen grains found in a glacier using a whole genome amplification method

*Fumio NAKAZAWA1, Yoshihisa SUYAMA2, Satoshi IMURA1, Hideaki MOTOYAMA1 (1.National Institute of Polar Research, 2.Tohoku University)

Keywords:glacier, ice core, pollen analysis, Pinus, DNA, WGA

Pollen taxon in sediment samples can be identified by analyzing pollen morphology. Identification of related species based on pollen morphology is difficult and is limited primarily to genus or family. Because many pollen grains in glaciers contain protoplasm, genetic information of pollen grains should enable identification of plant taxa below the genus level. The present study attempted to analyze the DNA of Pinus pollen grains collected from subsurface snow layers on the Belukha Glacier in the Altai Mountains of Russia in the summer of 2003 in order to identify them. Pinus is a taxon with approximately 111 recognized species in two subgenera, four sections and 11 subsections. Each Pinus pollen grain was amplified using a whole genome amplification method, and some regions of chloroplast genome were sequenced. As a result, each pollen grain was identified at subsection level and was narrowed down to around 10 species.