11:45 AM - 12:00 PM
[MIS31-10] Application of the transmission electron microscopy to the study of the crystallization process in a solution
We used two TEMs with LaB6 filament at an acceleration voltage of 200 kV (Hitachi H-8100) and with field-emission gun at an acceleration voltage of 300 kV (Hitachi HF-3300). For the observation of crystals in a solution, we used a “Poseidon” TEM holder (Protochip, Inc.) combined with a liquid cell. The liquid cell consists of a pair of semiconductor-based plates with an amorphous silicon nitride window and 150 or 500-nm-thick spacer to form the flow path of the crystallization solution. The lysozyme was crystallized using NaCl as a precipitant in a sodium acetate buffer solution at pH = 4.5.
As a result, we succeeded in observing the crystals and amorphous particles of lysozyme protein using the TEM and the liquid cell holder. In this presentation, we report the recent results of in situ observation of its crystallization process including the growth and dissolution.
We thank M. Shirai and H. Matsumoto of Hitachi High-Technologies Corporation for their help with the TEM observation using HF-3300. The authors acknowledge supports from a Grant-in-Aid for Research Activity Start-up from KAKENHI (26887001) and for a grant for Young Scientists (A) from KAKENHI (24684033).
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