10:00 AM - 10:15 AM
[ACC28-05] Temporal and spatial variations in pigment compositions of snow algae in Mt. Tateyama in Toyama prefecture, Japan
Keywords:Tateyama, Snow algae, Chlorophyll a, Astaxanthin, Carotenoid
Absorption spectrums of extracts from the colored snow showed that there were four absorption maximums in absorption spectrums. Each absorption maximums may correspond to pigments contained in the algae, including Chlorophyll a, Astaxanthin and unknown Carotenoid. Absorption spectrums varied among the samples, and that could be classified into 4 types: Type A (with maximums of Chlorophyll a and Astaxanthin), B (with maximums of Chlorophyll a and unknown Carotenoid), C (with maximums of Chlorophyll a only), and D (without any maximum). Microscopy of the samples revealed that the samples of A and B types contained snow algae of different color and structure: red sphere cells in Type A, and orange sphere, yellow sphere, green oblong cells in Type B. Analyses of the 18S rRNA gene identified 15 OTUs of algal gene in the samples. The samples of Type A and B contained distinctive OTUs of the algae, respectively, suggesting that the difference of algal pigments between Type A and B is not due to pigment compositions in same algal taxon, but to those of different algal taxa. Analysis of seasonal changes revealed that pigment compositions changed from Type A to Type B at the same location during the study period, suggesting that algal species composition on the snow surface change with time. The results also showed that the colored snow of Type A, B, and C appeared on several locations in Tateyama mountains from June to August. Variations in algal species and pigment compositions among time and locations may be attributed to life cycles and the dispersal of algae.