Japan Geoscience Union Meeting 2015

Presentation information

International Session (Oral)

Symbol M (Multidisciplinary and Interdisciplinary) » M-IS Intersection

[M-IS03] Exploring the role of soil in earth science: ecological/biogeochemical linkage and beyond

Wed. May 27, 2015 2:15 PM - 4:00 PM 104 (1F)

Convener:*Rota Wagai(National Institute for Agro-Environmental Sciences, Carbon & Nutrient Cycling Division), Kosaki Takashi(Tokyo Metropolitan University), Chair:Rota Wagai(National Institute for Agro-Environmental Sciences, Carbon & Nutrient Cycling Division)

2:30 PM - 2:45 PM

[MIS03-10] Carbon and nitrogen transformation and their driving microorganisms in paddy soil, as assessed by meta-transcriptomics

*Yoko TABUSHI1, Hideomi ITOH2, Yutaka SHIRATORI3, Kazuo ISOBE1, Shigeto OTSUKA1, Keishi SENOO1 (1.Graduate School of Agricultural and Life Sciences, The University of Tokyo, 2.National Institute of Advanced Industrial Science and Technology (AIST) Hokkaido, 3.Niigata Agricultural Research Institute)

Keywords:soil microbial communities, metatranscriptome, bioinformatics, paddy soil, CN cycle

Paddy soil undergoes drastic change in redox potential along water management during rice cultivation period. After waterlogging, various reductive biochemical processes, including denitrification, metal and sulfate reduction, and methanogenesis can occur in the soil, leading to generation of reduced soil layer. On the other hand, thin oxidized layer is present at soil surface, where oxidative biochemical processes such as nitrification and methane oxidation can occur. These oxidative and reductive reactions play central role in material transformation in paddy soil and are closely related to soil fertility, rice growth and surrounding environment.
We carried out meta-transcriptomic analysis of oxidized and reduced soils collected from Niigata paddy field during rice cultivation period. Whole microbial communities, quantity and diversity of transcriptome involved in carbon and nitrogen transformation in the soils were investigated by rRNA and mRNA analyses. Active microbial communities, possible carbon and nitrogen transformation, microbial players involved in the transformation, and their temporal transition in the paddy soil will be reported.