[BAO01-P09] Experimental study on role of sulfide-peptide complexes in the first metabolism
Keywords:Origin of life, Iron sulfide, Primitive enzyme, Iron-sulfur protein, Chemical evolution, Acetyl-CoA pathway
The catalytic activity of metal sulfides (FeS, Fe3S4, NiFe2S4, FeS2, CoS, NiS, and ZnS) for the conversion of CO2 or CO was assayed under various hydrothermal conditions (20–100°C, pH 4–10, 2–100 atm, with/without reductants). Metal sulfides containing Fe and/or Ni catalyzed CO2 reduction by H2 to afford formate. Among them, the greigite species (Fe3S4 and NiFe2S4) showed the highest catalytic activity. Similarly, greigite catalyzed CO reduction by HS- more efficiently than did any other metal sulfides tested, producing 2–6 mM acetate after 24 h reaction under 100°C and 2 atm. At higher reaction pressures, CO2 was predominantly reduced by H2 to methane, indicating dissolved gas concentration strongly influence reaction products. These results suggest that greigite has superior catalytic activity and plays a central role in establishing the first metabolism. In order to construct sulfide-peptide complexes, greigite-binding peptides were screened by using phage display random peptide libraries. After three rounds of biopanning, several consensus peptide sequences that were rich in polar amino acids (especially histidine) were obtained and found to attach to greigite. Preliminary experiments to study sulfide-peptide complexes are underway, and the results would be reported in due course.