[BAO01-P13] Mutation analysis of the rpoB gene in the radiation-resistant bacterium, Deinococcus radiodurans R1 exposed to space
Keywords:DNA damage, Deinococcus spp., International Space Station, Mutation, rpoB gene, Space
D. radiodurans R1 cell-suspension was dropped in the wells of aluminum plates and was dried under vacuum (vacuum-dried). The dried cells were exposed to space, stored in ISS cabin or in the ground laboratory. After exposure experiment, the cells recovered from each well were used to inoculate 10 ml of mTGE medium and cultured until OD590 nm reached between 0.7 and 3.0. The cell suspension was plated on mTGE agar containing 50μg/ml rifampicin to determine the number of rifampicin resistant cells (RifR), and on mTGE agar without rifampicin to determine the total number of viable cells.
We also determined the sequences of the rpoB gene extracted from RifR. The rifampicin resistant mutation frequencies of the space exposed D. radiodurans R1 cells and those of the ground control were comparable (Fig. 1). The result suggested that the effect of UV for dried-cells exposure to space was less. Further, we will report and discuss the rifampicin-resistant spectra in the rpoB gene in rifampicin-resistant cells exposure to space, stored in ISS or in ground laboratory.
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