12:00 PM - 12:15 PM
[BBG02-11] Synthesis of pyrite nanoparticles using organic molecules from Chrysomallon squamiferum
Keywords:Chrysomallon squamiferum, Heme proteins, pyrite nanoparticles
[Abstract] In this study, we analyzed the organic molecules extracted from the scaly structure and the shell in the scaly foot and searched for substances related to the production of iron sulfide nanoparticles by interaction with iron.
Pyrite in the scaly structure was dissolved by reducing with zinc and trivalent chromium under anaerobic conditions. We used HPLC post column chelator method to identify the iron binding organic molecules. This result revealed that large amount of low molecular weight organic matters having iron binding ability existed in the scaly structure. This low molecular weight organic molecule was purified using cation exchange resin and reverse phase-HPLC. We tried to determine its chemical structure using mass spectrometry and NMR.
On the other hand, 1 M acetic acid was used to dissolve the calcium carbonate layer of the outer shell. Then, the insoluble iron sulfide layer in acetic acid was extracted using SDS-DTT. A specific protein band was detected from the iron sulfide layer. LC-MS/MS analyses revealed that this band showed an amino sequence of heme protein. Heme is consisting of tetrapyrrole ring that can make the complex with iron in vivo.
The heme protein may have some roles for the formation of nano pyrite in vitro. We try to use the heme protein to synthesize pyrite nanoparticles using various conditions.