Japan Geoscience Union Meeting 2018

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[JJ] Poster

B (Biogeosciences) » B-BG Biogeosciences & Geosphere-Biosphere Interactions

[B-BG02] Interrelation between Life, Water, Mineral, and Atmosphere

Mon. May 21, 2018 1:45 PM - 3:15 PM Poster Hall (International Exhibition Hall7, Makuhari Messe)

convener:Ken Takai(Extremobiosphere Research Center, Japan Agency for Marine-Earth Science & Technology), Kentaro Nakamura(Department of Systems Innovation, School of Engineering, University of Tokyo), Yuichiro Ueno(東京工業大学大学院地球惑星科学専攻, 共同), Yohey Suzuki(Graduate School of Science, The University of Tokyo)

[BBG02-P12] Polymerization of metal oxide by silica-polymerizing enzyme

*Kazunori Nakashima1, Hidetoshi Oguri1, Junnosuke Okamoto1, Satoru Kawasaki1 (1.Hokkaido University)

Keywords:silica-polymerizing enzyme, biomineralization

Silicatein is an enzyme which was found in a glass skeleton of marine sponges and catalyzes the polymerization of silica under mild condition. Filament composed of silicatein isoforms, silicatein-alpha, silicatein-beta and silintaphin, can catalyze the polymerization of some kinds of metal oxide such as silica and titania. Silicatein-alpha, and silicatein-beta are aggregative proteins, which would cause a difficulty in engineering application of silicatein. We prepared silicatein-alpha and silicatein-beta fused with soluble protein to improve the solubility of these enzymes. These fusion proteins can be expressed in E. coli and are found to be stably soluble after refolding. The formation of silicatein filament can be controlled and the polymerization of silica and titania has been successfully achieved.