[BCG07-P04] 2D-GC/MS analysis of amino acids as pivaloyl / isopropyl esters: preliminary report
Keywords:Amino acids, 2D-GC/MS analysis, Pivaloyl / isopropyl esters
We review an analytical procedure using 1-dimensional (1D) and 2-dimensional (2D) gas chromatography / mass spectrometry (GC/MS) focusing on precise separations and the corresponding mass spectra of protein and non-protein amino acid derivatives. We used N-pivaloyl isopropyl esters of amino acids to show the summary of retention time and those of mass fragmentation patters [1,2,3]. The present method will be applied into a further description of biogeochemistry of individual amino acid.
Acknowledgement to F. Kabashima (LECO Japan) and M. Sakurai LECO Japan) in terms of the analytical development of multidimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC-TOFMS) analysis.
[ References ]
[1] Metges et al. (1996) Gas chromatography combustion isotope ratio mass spectrometric comparison of N-acetyl- and N-pivaloyl amino acid esters to measure N-15 isotopic abundances in physiological samples. Journal of Mass Spectrometry, 31, 367-376.
[2] Chikaraishi et al. (2009) Determination of aquatic food-web structure based on compound-specific nitrogen isotopic composition of amino acids. Limnology and Oceanography: Methods, 7, 740-750.
[3] Ohkouchi et al. (2017) Advances in the application of amino acid nitrogen isotopic analysis in ecological and biogeochemical studies. Organic Geochemistry, 113, 150-174.
[4] Takano et al. (2015) Isolation of underivatized amino acids by ion-pair high performance liquid chromatography for precise measurement of nitrogen isotopic composition of amino acids: development of comprehensive LC x GC/C/IRMS method. International Journal of Mass Spectrometry, 379, 16-25.
[5] Sugahara et al. (2018) The fate of D-amino acids in molecular evolution in space: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics, doi: 10.1016/j.bbapap.2018.01.004
Acknowledgement to F. Kabashima (LECO Japan) and M. Sakurai LECO Japan) in terms of the analytical development of multidimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC-TOFMS) analysis.
[ References ]
[1] Metges et al. (1996) Gas chromatography combustion isotope ratio mass spectrometric comparison of N-acetyl- and N-pivaloyl amino acid esters to measure N-15 isotopic abundances in physiological samples. Journal of Mass Spectrometry, 31, 367-376.
[2] Chikaraishi et al. (2009) Determination of aquatic food-web structure based on compound-specific nitrogen isotopic composition of amino acids. Limnology and Oceanography: Methods, 7, 740-750.
[3] Ohkouchi et al. (2017) Advances in the application of amino acid nitrogen isotopic analysis in ecological and biogeochemical studies. Organic Geochemistry, 113, 150-174.
[4] Takano et al. (2015) Isolation of underivatized amino acids by ion-pair high performance liquid chromatography for precise measurement of nitrogen isotopic composition of amino acids: development of comprehensive LC x GC/C/IRMS method. International Journal of Mass Spectrometry, 379, 16-25.
[5] Sugahara et al. (2018) The fate of D-amino acids in molecular evolution in space: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics, doi: 10.1016/j.bbapap.2018.01.004